HSD17B3 Rabbit Polyclonal Antibody

Ficha técnica

FISPQ

Key features and details

Rabbit polyclonal antibody to HSD17B3

Alvo: HSD17B3
Fonte/Hospedeiro: Coelho
Reatividade: Humano, Rato, Rato
Clonalidade: Policlonal
Aplicações: WB
Conjugação: Não conjugado
Armazenamento: a-20°C

Marca:

GATO.NÃO. : APA15859

US$ Por favor escolha

Tamanho:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detalhes do produto

Plano de fundo

Catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids . Favors the reduction of androstenedione to testosterone . Testosterone is the key androgen driving male development and function . Uses NADPH while the two other EDH17B enzymes use NADH . Androgens such as epiandrosterone, dehydroepiandrosterone, androsterone and androstanedione are accepted as substrates and reduced at C-17 . Can reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms .

Aplicação

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Visão geral

Descrição	Rabbit polyclonal antibody to HSD17B3
Especificidade	Recognizes endogenous levels of HSD17B3 protein
Tipo de anticorpo	Anticorpo primário
Imunogênio	Recombinant fusion protein of human HSD17B3. The exact sequence is proprietary.
Purificação	The antibody was purified by immunogen affinity chromatography.
Peso molecular	Predicted: 29; Observed: 34 kD
Formulário/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomes alternativos	EDH17B3; Testosterone 17-beta-dehydrogenase 3; 17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase
Símbolo Genético	HSD17B3
Gene Entrez	3293(Human); 15487(Mouse); 117182(Rat)
SwissProt	P37058(Human); P70385(Mouse); O54939(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dados

Western blot analysis of HSD17B3 expression in rat lung (A) whole cell lysates. (Predicted band size: 29; 34 kD; Observed band size: 34 kD)

Armazenamento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Perguntas frequentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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