Anticorpo Policlonal de Coelho HPS-4

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Principais recursos e detalhes

Anticorpo policlonal de coelho para HPS-4

Alvo: HPS-4
Fonte/Hospedeiro: Coelho
Reatividade: Humano, Rato
Clonalidade: Policlonal
Aplicações: BM, IF/ICC
Conjugação: Não conjugado
Armazenamento: a-20°C

Marca:

GATO.NÃO. : APA15851

US$ Por favor escolha

Tamanho:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detalhes do produto

Plano de fundo

Component of the BLOC-3 complex, a complex that acts as a guanine exchange factor (GEF) for RAB32 and RAB38, promotes the exchange of GDP to GTP, converting them from an inactive GDP-bound form into an active GTP-bound form. The BLOC-3 complex plays an important role in the control of melanin production and melanosome biogenesis and promotes the membrane localization of RAB32 and RAB38 for RAB32 and RAB38, promotes the exchange of GDP to GTP, converting them from an inactive GDP-bound form into an active GTP-bound form. The BLOC-3 complex plays an important role in the control of melanin production and melanosome biogenesis and promotes the membrane localization of RAB32 and RAB38 .

Aplicação

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000
SE/ICC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Visão geral

Descrição	Anticorpo policlonal de coelho para HPS-4
Especificidade	Recognizes endogenous levels of HPS-4 protein
Tipo de anticorpo	Anticorpo primário
Imunogênio	KLH-conjugated synthetic peptide of human HPS-4. The exact sequence is proprietary.
Purificação	The antibody was purified by immunogen affinity chromatography.
Peso molecular	Previsto: 58; Observado: 78 kD
Formulário/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomes alternativos	KIAA1667; Hermansky-Pudlak syndrome 4 protein; Light-ear protein homolog
Símbolo Genético	HPS4
Gene Entrez	89781(Humano); 192232 (Rato)
SwissProt	Q9NQG7(Humano); Q99KG7 (Rato)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dados

Western blot analysis of HPS-4 expression in Jurkat (A), mouse liver (B) whole cell lysates. (Predicted band size: 58; 76; 78 kD; Observed band size: 78 kD)

Immunofluorescent analysis of HPS-4 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Armazenamento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Perguntas frequentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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