GSTA5 Rabbit Polyclonal Antibody

Ficha técnica

FISPQ

Key features and details

Rabbit polyclonal antibody to GSTA5

Alvo: GSTA5
Fonte/Hospedeiro: Coelho
Reatividade: Humano
Clonalidade: Policlonal
Aplicações: WB
Conjugação: Não conjugado
Armazenamento: a-20°C

Marca:

CAT.NO. : APA19335

US$ Please choose

Tamanho:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Plano de fundo

The glutathione S-transferases (GST; EC 2.5.1.18) catalyze the conjugation of reduced glutathiones and a variety of electrophiles, including many known carcinogens and mutagens. The cytosolic GSTs belong to a large superfamily, with members located on different chromosomes. For additional information on GSTs, see GSTA1 (MIM 138359).

Aplicação

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Visão geral

Descrição	Rabbit polyclonal antibody to GSTA5
Especificidade	Recognizes endogenous levels of GSTA5 protein.
Tipo de anticorpo	Primary antibody
Imunogênio	KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human GSTA5. The exact sequence is proprietary.
Purificação	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 25 kD; Observed: 26 kD
Formulário/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	Glutathione S-transferase A5; GST class-alpha member 5; Glutathione S-transferase A5-5
Símbolo Genético	GSTA5
Gene Entrez	221357 (humano)
SwissProt	Q7RTV2 (humano)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dados

Western blot analysis of GSTA5 expression in human liver (A) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 26 kD)

Armazenamento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Perguntas frequentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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