GEN1 Rabbit Polyclonal Antibody
Ficha técnica
Key features and details
Rabbit polyclonal antibody to GEN1
- Alvo:
- Fonte/Hospedeiro:
- Reatividade:
- Clonalidade:
- Aplicações:
- Conjugação:
- Armazenamento:
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Marca:
GATO.NÃO. : APA13822
US$ Por favor escolha
US$ Por favor escolha
Detalhes do produto
Plano de fundo
Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation .
Aplicação
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:2000 |
Visão geral
Descrição | Rabbit polyclonal antibody to GEN1 |
Especificidade | Recognizes endogenous levels of GEN1 protein. |
Tipo de anticorpo | Anticorpo primário |
Imunogênio | Recombinant fusion protein of human GEN1 |
Purificação | The antibody was purified by immunogen affinity chromatography. |
Peso molecular | Predicted: 102 kD; Observed: 103 kD |
Formulário/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Nomes alternativos | Flap endonuclease GEN homolog 1 |
Símbolo Genético | GER1 |
Gene Entrez | 348654(Human); 209334(Mouse) |
SwissProt | Q17RS7(Human); Q8BMI4(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Dados
Western blot analysis of GEN1 expression in HL60 (A), MCF7 (B), mouse spleen (C) whole cell lysates. (Predicted band size: 102 kD; Observed band size: 103 kD)
Armazenamento
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Nota
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
Perguntas frequentes
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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