Anticorpo Policlonal de Coelho CTDP1

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Principais recursos e detalhes

Anticorpo policlonal de coelho para CTDP1

Alvo: CTDP1
Fonte/Hospedeiro: Coelho
Reatividade: Humano
Clonalidade: Policlonal
Aplicações: WB, IHC, FC
Conjugação: Não conjugado
Armazenamento: a-20°C

Marca:

GATO.NÃO. : APA18903

US$ Por favor escolha

Tamanho:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detalhes do produto

Plano de fundo

Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation.

Aplicação

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHQ	1:50 - 1:200
FC	1:10 - 1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Visão geral

Descrição	Anticorpo policlonal de coelho para CTDP1
Especificidade	Recognizes endogenous levels of CTDP1 protein.
Tipo de anticorpo	Anticorpo primário
Imunogênio	KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human CTDP1. The exact sequence is proprietary.
Purificação	The antibody was purified by immunogen affinity chromatography.
Peso molecular	Previsto: 104 kD; Observado: 104 kD
Formulário/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomes alternativos	FCP1; RNA polymerase II subunit A C-terminal domain phosphatase; TFIIF-associating CTD phosphatase
Símbolo Genético	CTDP1
Gene Entrez	9150 (Humano)
SwissProt	Q9Y5B0(Humano)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dados

Western blot analysis of CTDP1 expression in 293 (A), NCIH1299 (B) whole cell lysates. (Predicted band size: 104 kD; Observed band size: 104 kD)

Immunohistochemical analysis of CTDP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of WiDr cells using Anti-CTDP1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Armazenamento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Perguntas frequentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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