Anticorpo monoclonal de camundongo CREB (C3701)

Ficha técnica

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Principais recursos e detalhes

Anticorpo monoclonal de camundongo para CREB

Alvo: CREB
Fonte/Hospedeiro: Rato
Reatividade: Humano, Rato, Rato
Clonalidade: Monoclonal
Aplicações: WB
Conjugação: Não conjugado
Armazenamento: a-20°C

Marca:

GATO.NÃO. : AMA03313

US$ Por favor escolha

Tamanho:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detalhes do produto

Plano de fundo

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters . Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation , a sequence present in many viral and cellular promoters . Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation . Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells . Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling .

Aplicação

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHQ	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Visão geral

Descrição	Anticorpo monoclonal de camundongo para CREB
Especificidade	Recognizes endogenous levels of CREB protein.
Tipo de anticorpo	Anticorpo primário
Imunogênio	Recombinant fusion protein of human CREB. The exact sequence is proprietary.
Purificação	This antibody is purified through a protein G column.
Peso molecular	Previsto: 35 kD; Observado: 43 kD
Formulário/Buffer	Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nomes alternativos	Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1
Símbolo Genético	CREB1
Gene Entrez	1385(Humano); 12912(Rato); 81646 (Rato)
SwissProt	P16220(Human); Q01147(Mouse); P15337(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Dados

Western blot analysis of CREB expression in A549 (A), Neuro2a (B), NIH3T3 (C), C6 (D) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 43 kD)

Immunohistochemical analysis of CREB staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Armazenamento

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Nota

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

Perguntas frequentes

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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