ZIP9 Rabbit Polyclonal Antibody
MSDS
주요 기능 및 세부정보
Rabbit polyclonal antibody to ZIP9
- 대상:
- 소스/호스트:
- 반응성:
- 클론성:
- 신청:
- 활용:
- 저장:
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브랜드:
제품 세부정보
배경
Transports zinc ions across cell and organelle membranes into the cytoplasm and regulates intracellular zinc homeostasis . Participates in the zinc ions efflux out of the secretory compartments . Regulates intracellular zinc level, resulting in the enhancement of AKT1 and MAPK3/MAPK1 (Erk1/2) phosphorylation in response to the BCR activation . Also functions as a membrane androgen receptor that mediates, through a G protein, the non-classical androgen signaling pathway, characterized by the activation of MAPK3/MAPK1 (Erk1/2) and transcription factors CREB1 or ATF1 . This pathway contributes to CLDN1 and CLDN5 expression and tight junction formation between adjacent Sertoli cells .
신청
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
개요
설명 | Rabbit polyclonal antibody to ZIP9 |
특이성 | Recognizes endogenous levels of ZIP9 protein. |
항체 유형 | 1차 항체 |
면역원 | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZIP9. The exact sequence is proprietary. |
정화 | The antibody was purified by immunogen affinity chromatography. |
분자량 | Predicted: 32 kD; Observed: 33 kD |
형태/버퍼 | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
대체 이름 | ZIP9; Zinc transporter ZIP9; Solute carrier family 39 member 9; Zrt- and Irt-like protein 9; ZIP-9 |
유전자 기호 | SLC39A9 |
엔트레즈 진 | 55334(Human); 328133(Mouse); 314275(Rat) |
스위스프롯 | Q9NUM3(Human); Q8BFU1(Mouse); Q3KR82(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
데이터
Western blot analysis of ZIP9 expression in U937 (A), Raw264.7 (B), H9C2 (C) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 33 kD)
저장
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
참고
For Research Use Only. Not for use in diagnostic procedures.
자주 묻는 질문
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
신제품
Rabbit polyclonal antibody to SSX-pan
