WDFY4 Rabbit Polyclonal Antibody
MSDS
Key features and details
Rabbit polyclonal antibody to WDFY4
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브랜드:
CAT.NO. : APA17304
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제품 세부정보
배경
Plays a critical role in the regulation of cDC1-mediated cross-presentation of viral and tumor antigens in dendritic cells. Mechanistically, acts near the plasma membrane and interacts with endosomal membranes to promote endosomal-to-cytosol antigen trafficking. Also plays a role in B-cell survival through regulation of autophagy.
신청
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:200 |
개요
설명 | Rabbit polyclonal antibody to WDFY4 |
특이성 | Recognizes endogenous levels of WDFY4 protein |
항체 유형 | Primary antibody |
면역원 | Recombinant fusion protein of human WDFY4. The exact sequence is proprietary. |
정화 | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 73; Observed: 354 kD |
형태/버퍼 | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | C10orf64; KIAA1607; WD repeat- and FYVE domain-containing protein 4 |
유전자 기호 | WDFY4 |
엔트레즈 진 | 57705(인간) |
스위스프롯 | Q6ZS81(인간) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
데이터
Western blot analysis of WDFY4 expression in HeLa (A) whole cell lysates. (Predicted band size: 73; 114; 325; 352; 353 kD; Observed band size: 354 kD)
저장
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
참고
For Research Use Only. Not for use in diagnostic procedures.
자주 묻는 질문
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
신제품
Rabbit polyclonal antibody to SSX-pan
