PRRX1 토끼 다클론 항체

MSDS

주요 기능 및 세부정보

PRRX1에 대한 토끼 다클론 항체

대상: PRRX1
소스/호스트: 토끼
반응성: 인간, 생쥐, 쥐, 소, 닭
클론성: 다클론성
신청: WB, IF/ICC
활용: 비결합
저장: 에서-20°C

브랜드:

카탈로그 번호 : APA07408

US$ 선택하세요

크기:

50μL 100μL

트레일, 대량 크기 또는 맞춤형 요청 당사에 문의하십시오.

제품 세부정보

배경

Master transcription factor of stromal fibroblasts for myofibroblastic lineage progression. Orchestrates the functional drift of fibroblasts into myofibroblastic phenotype via TGF-beta signaling by remodeling a super-enhancer landscape. Through this function, plays an essential role in wound healing process . Acts as a transcriptional regulator of muscle creatine kinase (MCK) and so has a role in the establishment of diverse mesodermal muscle types. The protein binds to an A/T-rich element in the muscle creatine enhancer . May play a role in homeostasis and regeneration of bone, white adipose tissue and derm .

신청

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IF/ICC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

개요

설명	PRRX1에 대한 토끼 다클론 항체
특이성	PRRX1 단백질의 내인성 수준을 인식합니다.
항체 유형	1차 항체
면역원	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PRRX1. The exact sequence is proprietary.
정화	The antibody was purified by immunogen affinity chromatography.
분자량	예측: 27kD; 관찰된 사람: 27명; 24kD
형태/버퍼	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
대체 이름	PMX1; Paired mesoderm homeobox protein 1; Homeobox protein PHOX1; Paired-related homeobox protein 1; PRX-1
유전자 기호	PRRX1
엔트레즈 진	5396(인간); 18933(마우스); 266813(쥐)
스위스프롯	P54821(인간); P63013(마우스); P63014(쥐)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

데이터

Western blot analysis of PRRX1 expression in mouse spleen (A), rat ovary (B), Hela (C) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 27; 24 kD)

Immunofluorescent analysis of PRRX1 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

저장

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

참고

For Research Use Only. Not for use in diagnostic procedures.

자주 묻는 질문

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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