NCOA4 토끼 다클론항체

데이터시트

MSDS

주요 기능 및 세부정보

NCOA4에 대한 토끼 다클론 항체

대상: NCOA4
소스/호스트: 토끼
반응성: 인간
클론성: 다클론성
신청: WB, IHC, IF/ICC
활용: 비결합
저장: 에서-20°C

브랜드:

카탈로그 번호 : APA08737

US$ 선택하세요

크기:

50μL 100μL

트레일, 대량 크기 또는 맞춤형 요청 당사에 문의하십시오.

제품 세부정보

배경

Cargo receptor for the autophagic turnover of the iron-binding ferritin complex, playing a central role in iron homeostasis . Acts as an adapter for delivery of ferritin to lysosomes and autophagic degradation of ferritin, a process named ferritinophagy . Targets the iron-binding ferritin complex to autolysosomes following starvation or iron depletion . Ensures efficient erythropoiesis, possibly by regulating hemin-induced erythroid differentiation . In some studies, has been shown to enhance the androgen receptor AR transcriptional activity as well as acting as ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma .

신청

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:50 - 1:100
IF/ICC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

개요

설명	NCOA4에 대한 토끼 다클론 항체
특이성	NCOA4 단백질의 내인성 수준을 인식합니다.
항체 유형	1차 항체
면역원	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NCOA4. The exact sequence is proprietary.
정화	항체를 면역원 친화성 크로마토그래피로 정제하였다.
분자량	예상: 32; 관찰됨: 70kD
형태/버퍼	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
대체 이름	ARA70; ELE1; RFG; Nuclear receptor coactivator 4; NCoA-4; Androgen receptor coactivator 70 kDa protein; 70 kDa AR-activator; 70 kDa androgen receptor coactivator; Androgen receptor-associated protein of 70 kDa; Ret-activating protein ELE1
유전자 기호	NCOA4
엔트레즈 진	8031(인간)
스위스프롯	Q13772(인간)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

데이터

Western blot analysis of NCOA4 expression in H1688 (A) whole cell lysates. (Predicted band size: 32; 69; 71; 73 kD; Observed band size: 70 kD)

Immunohistochemical analysis of NCOA4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of NCOA4 staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

저장

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

참고

연구용으로만 사용하세요. 진단, 치료, 예방 또는 생체 내 사용을 위한 것이 아닙니다.

자주 묻는 질문

연구용 항체의 주요 유형은 무엇이며 어떻게 다른가요?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

연구 항체가 내 실험에 적합한지 어떻게 알 수 있나요?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

연구용 항체를 부적절하게 보관하면 실험 결과에 영향을 미칠 수 있나요?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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