Glucocorticoid Receptor Mouse Monoclonal Antibody(C3593)
데이터시트
주요 기능 및 세부정보
Mouse monoclonal antibody to Glucocorticoid Receptor
- 대상:
- 소스/호스트:
- 반응성:
- 클론성:
- 신청:
- 활용:
- 저장:
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브랜드:
제품 세부정보
배경
Receptor for glucocorticoids (GC) . Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors . Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling . Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay . Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth .
신청
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
개요
설명 | Mouse monoclonal antibody to Glucocorticoid Receptor |
특이성 | Recognizes endogenous levels of Glucocorticoid Receptor protein. |
항체 유형 | 1차 항체 |
면역원 | Recombinant fusion protein of human Glucocorticoid Receptor. The exact sequence is proprietary. |
정화 | This antibody is purified through a protein G column. |
분자량 | Predicted: 85 kD; Observed: 94 kD |
형태/버퍼 | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
대체 이름 | GRL; Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1 |
유전자 기호 | NR3C1 |
엔트레즈 진 | 2908(인간) |
스위스프롯 | P04150(인간); P06537(마우스) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
데이터
Western blot analysis of Glucocorticoid Receptor expression in HeLa (A), A549 (B), CCRFCEM (C), CHO (D) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 94 kD)
Western blot analysis of Glucocorticoid Receptor expression in wild type (WT) and knockdown (KD) HeLa cell lysates.
저장
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
참고
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
자주 묻는 질문
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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