CREB3L1 토끼 다클론항체

MSDS

주요 기능 및 세부정보

CREB3L1에 대한 토끼 다클론 항체

대상: CREB3L1
소스/호스트: 토끼
반응성: 인간, 쥐
클론성: 다클론성
신청: WB, IHC, FC
활용: 비결합
저장: 에서-20°C

브랜드:

카탈로그 번호 : APA18900

US$ 선택하세요

크기:

50μL 100μL

트레일, 대량 크기 또는 맞춤형 요청 당사에 문의하십시오.

제품 세부정보

배경

Precursor of the transcription factor form (Processed cyclic AMP-responsive element-binding protein 3-like protein 1), which is embedded in the endoplasmic reticulum membrane with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane , which is embedded in the endoplasmic reticulum membrane with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane . In response to ER stress or DNA damage, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2.

신청

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:1000 - 1:2000
IHC	1:50 - 1:200
FC	1:10 - 1시 50분

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

개요

설명	CREB3L1에 대한 토끼 다클론 항체
특이성	CREB3L1 단백질의 내인성 수준을 인식합니다.
항체 유형	1차 항체
면역원	KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human CREB3L1. The exact sequence is proprietary.
정화	항체를 면역원 친화성 크로마토그래피로 정제하였다.
분자량	예측: 57kD; 관찰된 수: 57; 48kD
형태/버퍼	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
대체 이름	OASIS; Cyclic AMP-responsive element-binding protein 3-like protein 1; cAMP-responsive element-binding protein 3-like protein 1; Old astrocyte specifically-induced substance; OASIS
유전자 기호	CREB3L1
엔트레즈 진	90993(인간); 26427(마우스)
스위스프롯	Q96BA8(인간); Q9Z125(마우스)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

데이터

Western blot analysis of CREB3L1 expression in HepG2 (A) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 57; 48 kD)

Immunohistochemical analysis of CREB3L1 staining in human prostata carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of Hela cells using Anti-CREB3L1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

저장

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

참고

연구용으로만 사용됩니다. 진단 절차에 사용하지 마십시오.

자주 묻는 질문

연구용 항체의 주요 유형은 무엇이며 어떻게 다른가요?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

연구 항체가 내 실험에 적합한지 어떻게 알 수 있나요?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

연구용 항체를 부적절하게 보관하면 실험 결과에 영향을 미칠 수 있나요?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

데이터시트에 있는 권장 희석액이 내 실험에서 잘 작동하지 않는 이유는 무엇입니까?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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