BRD7 Rabbit Polyclonal Antibody
MSDS
주요 기능 및 세부정보
Rabbit polyclonal antibody to BRD7
- 대상:
- 소스/호스트:
- 반응성:
- 클론성:
- 신청:
- 활용:
- 저장:
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브랜드:
제품 세부정보
배경
Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR . Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation.
신청
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:2000 |
개요
설명 | Rabbit polyclonal antibody to BRD7 |
특이성 | Recognizes endogenous levels of BRD7 protein. |
항체 유형 | 1차 항체 |
면역원 | Recombinant fusion protein of human BRD7 |
정화 | The antibody was purified by immunogen affinity chromatography. |
분자량 | Predicted: 74 kD; Observed: 100 kD |
형태/버퍼 | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
대체 이름 | BP75; CELTIX1; Bromodomain-containing protein 7; 75 kDa bromodomain protein; Protein CELTIX-1 |
유전자 기호 | BRD7 |
엔트레즈 진 | 29117(인간); 26992(마우스) |
스위스프롯 | Q9NPI1(Human); O88665(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
데이터
Western blot analysis of BRD7 expression in HepG2 (A), SW620 (B) whole cell lysates. (Predicted band size: 74 kD; Observed band size: 100 kD)
저장
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
참고
For Research Use Only. Not for use in diagnostic procedures.
자주 묻는 질문
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
신제품
Rabbit polyclonal antibody to SSX-pan
