p47 phox (Phospho-S304) Antibodi Poliklonal Kelinci

MSDS

p47 phox (Phospho-S304) Rabbit Polyclonal Antibody

Fitur dan detail utama

Antibodi poliklonal kelinci terhadap p47 phox (Phospho-S304)

Sasaran: p47 foto (Fosfo-S304)
Sumber/Pembawa Acara: Kelinci
Reaktivitas: Manusia, Tikus, Tikus, Sapi, Anjing, Domba
Klonalitas: Poliklonal
Aplikasi: WB, IHC
Konjugasi: Tidak terkonjugasi
Penyimpanan: pada-20°C

Merek:

KUCING.TIDAK. : APA09408

US$ Silakan pilih

Ukuran:

50μL 100μL

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Detail Produk

Latar Belakang

Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) ) . In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction . Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane . This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox .

Aplikasi

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Ikhtisar

Deskripsi	Antibodi poliklonal kelinci terhadap p47 phox (Phospho-S304)
Kekhususan	Mengenali kadar protein p47 phox endogen hanya ketika terfosforilasi pada S304.
Tipe Antibodi	Antibodi primer
Imunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S304 of human p47 phox protein. The exact sequence is proprietary.
Pemurnian	Antibodi dimurnikan dengan kromatografi afinitas imunogen.
Berat Molekul	Prediksi: 44 kD; Teramati : 47 kD
Formulir/Penyangga	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nama Alternatif	NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox
Simbol Gen	NCF1
Entrez Gene	653361(Manusia)
SwissProt	P14598(Manusia)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of p47 phox (Phospho-S304) expression in HEK293T (A), Hela (B), mouse lung (C), mouse liver (D), rat liver (E), rat spleen (F) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 47 kD)

Immunohistochemical analysis of p47 phox (Phospho-S304) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Penyimpanan

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Catatan

Hanya Untuk Penggunaan Penelitian. Tidak untuk digunakan dalam prosedur diagnostik.

FAQ

Apa jenis utama antibodi penelitian dan apa perbedaannya?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

Bagaimana saya dapat mengetahui apakah antibodi penelitian cocok untuk eksperimen saya?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Apakah penyimpanan antibodi penelitian yang tidak tepat dapat mempengaruhi hasil eksperimen?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Mengapa pengenceran yang disarankan dalam lembar data tidak berfungsi dengan baik dalam eksperimen saya?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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