Kit ELISA Tikus IL-2

Interleukin 2 (IL-2), juga dikenal sebagai faktor pertumbuhan sel T (TCGF), adalah polipeptida alfa -heliks glikosilasi bervariasi 15-18 kDa yang merupakan anggota keluarga sitokin Common gamma Chain (gamma c). Ia ada sebagai monomer dan mempunyai waktu paruh pendek (< 30 minutes). Human IL-2 is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 133 aa mature region. The mature region is alpha -helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity. Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gamma δ T cells, activated conventional CD4+ and CD8+ T cells, neurons, microglia, and hematopoietic stem cells. The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain. IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gamma c to form the quaternary signaling complex. In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gamma c also serves as a signaling receptor for IL-4, -7, -9, -15, and -21. In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells.

hal/ml	OD.		Rata-rata	Dikoreksi
0.00	0.0100	0.0090	0.0095
2.74	0.0182	0.0182	0.0182	0.0087
8.23	0.0310	0.0310	0.0310	0.0215
24.69	0.0720	0.0736	0.0728	0.0633
74.07	0.1837	0.1918	0.1878	0.1783
222.22	0.5283	0.5149	0.5216	0.5121
666.67	1.4151	1.4413	1.4282	1.4187
2000.00	3.4657	3.4090	3.4374	3.4279

	Presisi Intra - pengujian			Presisi Antar-pengujian
Nomor Sampel	S1	S2	S3	S1	S2	S3
	22	22	22	6	6	6
Rata-rata (pg/ml)	43.5	121.4	362	18.9	152.5	341.9
Deviasi Standar	2.2	6.0	19.4	0.4	2.8	12.5
Koefisien Variasi (%)	5.1	5.0	5.4	2.1	1.8	3.6

Presisi Intra-assay (Presisi dalam suatu pengujian) Tiga sampel dengan konsentrasi yang diketahui diuji dua puluh kali pada satu pelat untuk menilai presisi intra-assay.

Presisi Antar-pengujian (Presisi antar pengujian) Tiga sampel dengan konsentrasi yang diketahui diuji enam kali pada satu pelat untuk menilai presisi intra-pengujian.

Pemulihan lonjakan dievaluasi dengan memasukkan 3 level IL-2 tikus ke dalam sampel serum tikus sehat. Serum yang tidak diberi duri digunakan sebagai blanko dalam percobaan ini.
Pemulihan berkisar antara 81% hingga 108% dengan rata-rata pemulihan keseluruhan sebesar 94%.

Contoh Matriks	Sampel Dievaluasi	Kisaran (hal/ml)	Terdeteksi (%)	Rata-rata Terdeteksi (pg/ml)
serum	30	nd-0,67	21	0.23

Serum/Plasma – Tiga puluh sampel dari tikus yang tampak sehat dievaluasi keberadaan IL-2 dalam pengujian ini. Tidak ada riwayat medis yang tersedia untuk para donor.

n.d. = tidak-terdeteksi. Sampel yang diukur di bawah sensitivitas dianggap tidak terdeteksi.