Kinase Adhesi Fokus (Phospho-Y407) Antibodi Poliklonal Kelinci

MSDS

Focal Adhesion Kinase (Phospho-Y407) Rabbit Polyclonal Antibody

Fitur dan detail utama

Antibodi poliklonal kelinci terhadap Focal Adhesion Kinase (Phospho-Y407)

Sasaran: Kinase Adhesi Fokus (Phospho-Y407)
Sumber/Pembawa Acara: Kelinci
Reaktivitas: Manusia, Tikus, Tikus, Ayam, Monyet, Ikan Zebra
Klonalitas: Poliklonal
Aplikasi: WB, IHC
Konjugasi: Tidak terkonjugasi
Penyimpanan: pada-20°C

Merek:

KUCING.TIDAK. : APA11971

US$ Silakan pilih

Ukuran:

50μL 100μL

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Detail Produk

Latar Belakang

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts.

Aplikasi

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Ikhtisar

Deskripsi	Antibodi poliklonal kelinci terhadap Focal Adhesion Kinase (Phospho-Y407)
Kekhususan	Recognizes endogenous levels of Focal Adhesion Kinase protein only when phosphorylated at Y407.
Tipe Antibodi	Antibodi primer
Imunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y407 of human Focal Adhesion Kinase protein. The exact sequence is proprietary.
Pemurnian	Antibodi dimurnikan dengan kromatografi afinitas imunogen.
Berat Molekul	Prediksi: 119; Teramati : 125 kD
Formulir/Penyangga	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nama Alternatif	FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
Simbol Gen	PTK2
Entrez Gene	5747(Manusia); 14083(Tikus); 25614 (Tikus)
SwissProt	Q05397(Manusia); P34152(Tikus); O35346(Tikus)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Focal Adhesion Kinase (Phospho-Y407) expression in HEK293T (A), Raw264.7 (B), rat heart (C) whole cell lysates. (Predicted band size: 119; 99; 63; 48; 120; 39; 114 kD; Observed band size: 125 kD)

Immunohistochemical analysis of Focal Adhesion Kinase (Phospho-Y407) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Penyimpanan

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Catatan

Hanya Untuk Penggunaan Penelitian. Tidak untuk digunakan dalam prosedur diagnostik.

FAQ

Apa jenis utama antibodi penelitian dan apa perbedaannya?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

Bagaimana saya dapat mengetahui apakah antibodi penelitian cocok untuk eksperimen saya?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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