Antibodi Poliklonal Kelinci FKBP6

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Antibodi poliklonal kelinci terhadap FKBP6

Sasaran: FKBP6
Sumber/Pembawa Acara: Kelinci
Reaktivitas: Manusia, Tikus, Tikus
Klonalitas: Poliklonal
Aplikasi: WB, JIKA/ICC
Konjugasi: Tidak terkonjugasi
Penyimpanan: pada-20°C

Merek:

KUCING.TIDAK. : APA13027

US$ Silakan pilih

Ukuran:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detail Produk

Latar Belakang

Has an essential role in spermatogenesis . It is required to repress transposable elements and prevent their mobilization, which is essential for the germline integrity . Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons . Acts as a co-chaperone via its interaction with HSP90 and is required for the piRNA amplification process, the secondary piRNA biogenesis . May be required together with HSP90 in removal of 16 nucleotide ping-pong by-products from Piwi complexes, possibly facilitating turnover of Piwi complexes .

Aplikasi

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
JIKA/ICC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Ikhtisar

Deskripsi	Antibodi poliklonal kelinci terhadap FKBP6
Kekhususan	Recognizes endogenous levels of FKBP6 protein.
Tipe Antibodi	Antibodi primer
Imunogen	Protein fusi rekombinan FKBP6 manusia
Pemurnian	The antibody was purified by immunogen affinity chromatography.
Berat Molekul	Prediksi: 33; Teramati : 37 kD
Formulir/Penyangga	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nama Alternatif	FKBP36; Inactive peptidyl-prolyl cis-trans isomerase FKBP6; Inactive PPIase FKBP6; 36 kDa FK506-binding protein; 36 kDa FKBP; FKBP-36; FK506-binding protein 6; FKBP-6; Immunophilin FKBP36
Simbol Gen	FKBP6
Entrez Gene	8468(Manusia); 94244(Tikus); 288597(Tikus)
SwissProt	O75344(Manusia); Q91XW8(Tikus); D3ZQF4(Tikus)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of FKBP6 expression in HepG2 (A), mouse heart (B), mouse liver (C), rat testis (D) whole cell lysates. (Predicted band size: 33; 36; 37 kD; Observed band size: 37 kD)

Immunofluorescent analysis of FKBP6 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Penyimpanan

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Catatan

For Research Use Only. Not for use in diagnostic procedures.

FAQ

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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