Antibodi Poliklonal Kelinci CD181

MSDS

Fitur dan detail utama

Rabbit polyclonal antibody to CD181

Sasaran: CD181
Sumber/Pembawa Acara: Kelinci
Reaktivitas: Manusia
Klonalitas: Poliklonal
Aplikasi: WB, JIKA/ICC
Konjugasi: Tidak terkonjugasi
Penyimpanan: pada-20°C

Merek:

KUCING.TIDAK. : APA11636

US$ Silakan pilih

Ukuran:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detail Produk

Latar Belakang

Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor . Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system .

Aplikasi

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
JIKA/ICC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Ikhtisar

Deskripsi	Rabbit polyclonal antibody to CD181
Kekhususan	Recognizes endogenous levels of CD181 protein.
Tipe Antibodi	Antibodi primer
Imunogen	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD181. The exact sequence is proprietary.
Pemurnian	The antibody was purified by immunogen affinity chromatography.
Berat Molekul	Prediksi : 39 kD; Diamati: 55 kD
Formulir/Penyangga	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nama Alternatif	CMKAR1; IL8RA; C-X-C chemokine receptor type 1; CXC-R1; CXCR-1; CDw128a; High affinity interleukin-8 receptor A; IL-8R A; IL-8 receptor type 1; CD181
Simbol Gen	CXCR1
Entrez Gene	3577 (Manusia)
SwissProt	P25024 (Manusia)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of CD181 expression in HEK293T (A), HuT78 (B), H1688 (C) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 55 kD)

Immunofluorescent analysis of CD181 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Penyimpanan

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Catatan

For Research Use Only. Not for use in diagnostic procedures.

FAQ

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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