CD131 Rabbit Polyclonal Antibody
MSDS
Key features and details
Rabbit polyclonal antibody to CD131
- Sasaran:
- Sumber/Pembawa Acara:
- Reaktivitas:
- Klonalitas:
- Aplikasi:
- Konjugasi:
- Penyimpanan:
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Merek:
KUCING.TIDAK. : APA06850
US$ Silakan pilih
US$ Silakan pilih
Detail Produk
Latar Belakang
Cell surface receptor that plays a role in immune response and controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Acts by forming an heterodimeric receptor through interaction with different partners such as IL3RA, IL5RA or CSF2RA . In turn, participates in various signaling pathways including interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor/CSF2 pathways. In unstimulated conditions, interacts constitutively with JAK1 and ligand binding leads to JAK1 stimulation and subsequent activation of the JAK-STAT pathway .
Aplikasi
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
Ikhtisar
Deskripsi | Rabbit polyclonal antibody to CD131 |
Kekhususan | Recognizes endogenous levels of CD131 protein. |
Tipe Antibodi | Antibodi primer |
Imunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD131. The exact sequence is proprietary. |
Pemurnian | The antibody was purified by immunogen affinity chromatography. |
Berat Molekul | Predicted: 97 kD; Observed: 130 kD |
Formulir/Penyangga | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Nama Alternatif | IL3RB; IL5RB; Cytokine receptor common subunit beta; CDw131; GM-CSF/IL-3/IL-5 receptor common beta subunit; CD131 |
Simbol Gen | CSF2RB |
Entrez Gene | 1439 (Manusia) |
SwissProt | P32927(Manusia) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of CD131 expression in HL60 (A) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 130 kD)
Penyimpanan
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Catatan
For Research Use Only. Not for use in diagnostic procedures.
FAQ
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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