Antibodi Monoklonal Tikus AKT (C2501)

MSDS

Fitur dan detail utama

Mouse monoklonal ke AKT

Sasaran: AKT
Sumber/Pembawa Acara: Tikus
Reaktivitas: Manusia, Tikus, Tikus, Monyet
Klonalitas: Monoklonal
Aplikasi: WB, IF/ICC, FC
Konjugasi: Tidak terkonjugasi
Penyimpanan: pada-20°C

Merek:

KUCING.TIDAK. :AMA02113

US$ Silakan pilih

Ukuran:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Detail Produk

Latar Belakang

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis . This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates . Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported . AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface .

Aplikasi

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
JIKA/ICC	1:100 - 1:500
FC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Ikhtisar

Deskripsi	Mouse monoklonal ke AKT
Kekhususan	Mengenali kadar protein AKT endogen
Tipe Antibodi	Antibodi primer
Imunogen	Recombinant fusion protein of human AKT expressed in E. Coli
Pemurnian	This antibody is purified through a protein G column.
Berat Molekul	Prediksi: 56 kD; Teramati : 55 kD kD
Formulir/Penyangga	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Nama Alternatif	PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
Simbol Gen	AKT1
Entrez Gene	207(Manusia); 11651(Tikus); 24185 (Tikus)
SwissProt	P31749(Manusia); P31750(Tikus); P47196(Tikus)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of AKT expression in MCF7 (A), NIH/3T3 (B), Hela (C), COS7 (D), C6 (E) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 55 kD kD)

Immunofluorescent analysis of AKT staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of HeLa cells using Anti-AKT Antibody (green) and negative control (red).

Penyimpanan

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Catatan

For Research Use Only. Not for use in diagnostic procedures.

FAQ

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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