ZSCAN32 Rabbit Polyclonal Antibody
Sicherheitsdatenblatt
Hauptmerkmale und Details
- Ziel:
- Quelle/Host:
- Reaktivität:
- Klonalität:
- Anwendungen:
- Konjugation:
- Lagerung:
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Marke:
Produktdetails
WB | 1:500 - 1:1000 |
IF/ICC | 1:10 - 1:50 |
Beschreibung | Rabbit polyclonal antibody to ZSCAN32 |
Spezifität | Recognizes endogenous levels of ZSCAN32 protein. |
Antikörpertyp | Primärer Antikörper |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human ZSCAN32. The exact sequence is proprietary. |
Reinigung | The antibody was purified by immunogen affinity chromatography. |
Molekulargewicht | Predicted: 78 kD; Observed: 55 kD |
Form/Puffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Namen | ZNF434; Zinc finger and SCAN domain-containing protein 32; Human cervical cancer suppressor gene 5 protein; HCCS-5; Zinc finger protein 434 |
Gensymbol | ZSCAN32 |
Entrez Gene | 54925 (Mensch) |
SwissProt | Q9NX65 (Mensch) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of ZSCAN32 expression in Jurkat (A), MCF7 (B) whole cell lysates. (Predicted band size: 78 kD; Observed band size: 55 kD)
Immunofluorescent analysis of Anti-ZSCAN32 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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