USP25 Polyklonaler Kaninchen-Antikörper

Sicherheitsdatenblatt

Hauptmerkmale und Details

Polyklonaler Kaninchen-Antikörper gegen USP25

Ziel: USP25
Quelle/Host: Kaninchen
Reaktivität: Mensch, Maus, Ratte
Klonalität: Polyklonal
Anwendungen: WB, IHC
Konjugation: Unkonjugiert
Lagerung: bei -20°C

Marke:

KAT.-NR. : APA13200

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50μL 100 μL

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Produktdetails

Hintergrund

Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions in various biological processes including inflammation and immune response . Modulates the Wnt/beta-catenin pathway by deubiquitinating and stabilizing tankyrases TNKS1 and TNKS2 . Regulates KEAP1-NRF2 axis in the defense against oxidative assaults by deubiquitinating KEAP1 and protecting it from degradation leading to degradation of the NRF2 transcription factor that is responsible for mounting an anti-oxidation gene expression program . Positively regulates RNA virus-induced innate signaling by interacting with and deubiquitinating ERLIN1 and ERLIN2 . In turn, restricts virus production by regulating cholesterol biosynthetic flux .

Bewerbung

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000
IHC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Übersicht

Beschreibung	Polyklonaler Kaninchen-Antikörper gegen USP25
Spezifität	Erkennt endogene Mengen an USP25-Protein.
Antikörpertyp	Primärer Antikörper
Immunogen	Rekombinantes Fusionsprotein des menschlichen USP25
Reinigung	The antibody was purified by immunogen affinity chromatography.
Molekulargewicht	Vorhergesagt: 122; Beobachtet: 130 kD
Form/Puffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Namen	USP21; Ubiquitin carboxyl-terminal hydrolase 25; Deubiquitinating enzyme 25; USP on chromosome 21; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25
Gensymbol	USP25
Entrez Gene	29761 (Mensch); 30940(Maus)
SwissProt	Q9UHP3 (Mensch); P57080 (Maus)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Daten

Western blot analysis of USP25 expression in SKOV3 (A), PC12 (B), mouse brain (C), mouse liver (D) whole cell lysates. (Predicted band size: 122; 125; 129 kD; Observed band size: 130 kD)

Immunohistochemical analysis of USP25 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Lagerung

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Hinweis

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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