TC-PTP Polyklonaler Kaninchen-Antikörper

Sicherheitsdatenblatt

Hauptmerkmale und Details

Polyklonaler Kaninchen-Antikörper gegen TC-PTP

Ziel: TC-PTP
Quelle/Host: Kaninchen
Reaktivität: Mensch, Maus, Ratte
Klonalität: Polyklonal
Anwendungen: WB, IF/ICC
Konjugation: Unkonjugiert
Lagerung: bei -20°C

Marke:

KAT.-NR. : APA13655

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Größe:

50μL 100 μL

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Produktdetails

Hintergrund

Non-receptor type tyrosine-specific phosphatase that dephosphorylates receptor protein tyrosine kinases including INSR, EGFR, CSF1R, PDGFR. Also dephosphorylates non-receptor protein tyrosine kinases like JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3 and STAT6 either in the nucleus or the cytoplasm. Negatively regulates numerous signaling pathways and biological processes like hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. Plays a multifaceted and important role in the development of the immune system. Functions in T-cell receptor signaling through dephosphorylation of FYN and LCK to control T-cells differentiation and activation.

Bewerbung

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IF/ICC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Übersicht

Beschreibung	Polyklonaler Kaninchen-Antikörper gegen TC-PTP
Spezifität	Recognizes endogenous levels of TC-PTP protein.
Antikörpertyp	Primärer Antikörper
Immunogen	Rekombinantes Fusionsprotein von menschlichem TC-PTP
Reinigung	The antibody was purified by immunogen affinity chromatography.
Molekulargewicht	Voraussichtlich: 40; Beobachtet: 48 kD
Form/Puffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Namen	PTPT; Tyrosine-protein phosphatase non-receptor type 2; T-cell protein-tyrosine phosphatase; TCPTP
Gensymbol	PTPN2
Entrez Gene	5771 (Mensch); 19255(Maus); 117063(Ratte)
SwissProt	P17706 (Mensch); Q06180(Maus); P35233(Ratte)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Daten

Western blot analysis of TC-PTP expression in Jurkat (A), K562 (B) whole cell lysates. (Predicted band size: 40; 45; 48 kD; Observed band size: 48 kD)

Immunofluorescent analysis of TC-PTP staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Lagerung

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Hinweis

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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