Polyklonaler SYK-Kaninchen-Antikörper

Sicherheitsdatenblatt

Hauptmerkmale und Details

Polyklonaler Kaninchen-Antikörper gegen SYK

Ziel: SYK
Quelle/Host: Kaninchen
Reaktivität: Mensch, Maus
Klonalität: Polyklonal
Anwendungen: WB, IHC, IF/ICC
Konjugation: Unkonjugiert
Lagerung: bei -20°C

Marke:

KAT.-NR. : APA10957

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Größe:

50μL 100 μL

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Produktdetails

Hintergrund

Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development . Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development . Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains.

Bewerbung

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

IHC	1:50 - 1:100
IF/ICC	1:100 - 1:500

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Übersicht

Beschreibung	Polyklonaler Kaninchen-Antikörper gegen SYK
Spezifität	Erkennt endogene Mengen an SYK-Protein.
Antikörpertyp	Primärer Antikörper
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SYK. The exact sequence is proprietary.
Reinigung	The antibody was purified by immunogen affinity chromatography.
Molekulargewicht	Voraussichtlich: 72 kD; Beobachtet: 72 kD
Form/Puffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Namen	Tyrosin-Proteinkinase SYK; Milz-Tyrosinkinase; p72-Syk
Gensymbol	SYK
Entrez Gene	6850 (Mensch); 20963(Maus)
SwissProt	P43405 (Mensch); P48025(Maus)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Daten

Western blot analysis of SYK expression in CT26 (A) whole cell lysates. (Predicted band size: 72 kD; Observed band size: 72 kD)

Immunohistochemical analysis of SYK staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of SYK staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Lagerung

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Hinweis

Nur für Forschungszwecke. Nicht zur Verwendung in diagnostischen Verfahren geeignet.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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