RPA4 Rabbit Polyclonal Antibody
Sicherheitsdatenblatt
Key features and details
Rabbit polyclonal antibody to RPA4
- Ziel:
- Quelle/Host:
- Reaktivität:
- Klonalität:
- Anwendungen:
- Konjugation:
- Lagerung:
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Marke:
CAT.NO. : APA09057
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Produktdetails
Hintergrund
As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Bewerbung
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
Übersicht
Beschreibung | Rabbit polyclonal antibody to RPA4 |
Spezifität | Recognizes endogenous levels of RPA4 protein. |
Antikörpertyp | Primärer Antikörper |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RPA4. The exact sequence is proprietary. |
Reinigung | The antibody was purified by immunogen affinity chromatography. |
Molekulargewicht | Predicted: 28 kD; Observed: 29 kD |
Form/Puffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Namen | Replication protein A 30 kDa subunit; RP-A p30; Replication factor A protein 4; RF-A protein 4 |
Gensymbol | RPA4 |
Entrez Gene | 29935 (Mensch) |
SwissProt | Q13156 (Mensch) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Daten
Western blot analysis of RPA4 expression in HEK293T (A), Raw264.7 (B), PC12 (C) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 29 kD)
Lagerung
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Hinweis
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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