PARG Rabbit Polyclonal Antibody
Sicherheitsdatenblatt
Key features and details
Rabbit polyclonal antibody to PARG
- Ziel:
- Quelle/Host:
- Reaktivität:
- Klonalität:
- Anwendungen:
- Konjugation:
- Lagerung:
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Marke:
KAT.-NR. : APA13973
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Produktdetails
Hintergrund
Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose) . Mainly acts as an exo-glycohydrolase but can act as an endo-glycohydrolase at low efficiency, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers . It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated . Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by PARG .
Bewerbung
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:2000 |
Übersicht
Beschreibung | Rabbit polyclonal antibody to PARG |
Spezifität | Recognizes endogenous levels of PARG protein. |
Antikörpertyp | Primärer Antikörper |
Immunogen | Recombinant fusion protein of human PARG |
Reinigung | The antibody was purified by immunogen affinity chromatography. |
Molekulargewicht | Predicted: 54; Observed: 111 kD |
Form/Puffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Namen | Poly; ADP-ribose) glycohydrolase |
Gensymbol | PARG |
Entrez Gene | 8505(Human); 83507(Rat) |
SwissProt | Q86W56(Human); O88622(Mouse); Q9QYM2(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Daten
Western blot analysis of PARG expression in K562 (A), Hela (B), NIH3T3 (C), mouse liver (D) whole cell lysates. (Predicted band size: 54; 60; 99; 102; 111 kD; Observed band size: 111 kD)
Lagerung
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Hinweis
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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