Polyklonaler Kaninchen-Antikörper Ku70
Sicherheitsdatenblatt
Hauptmerkmale und Details
- Ziel:
- Quelle/Host:
- Reaktivität:
- Klonalität:
- Anwendungen:
- Konjugation:
- Lagerung:
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Marke:
Produktdetails
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:100 |
IF/ICC | 1:50 - 1:200 |
Beschreibung | Polyklonaler Kaninchen-Antikörper gegen Ku70 |
Spezifität | Erkennt endogene Mengen an Ku70-Protein. |
Antikörpertyp | Primärer Antikörper |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Ku70. The exact sequence is proprietary. |
Reinigung | Der Antikörper wurde durch Immunogenaffinitätschromatographie gereinigt. |
Molekulargewicht | Voraussichtlich: 69 kD; Beobachtet: 70 kD |
Form/Puffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Namen | G22P1; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6 |
Gensymbol | XRCC6 |
Entrez Gene | 2547 (Mensch) |
SwissProt | P12956 (Mensch) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Ku70 expression in HEK293T (A), Hela (B), A2780 (C), H460 (D) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 70 kD)
Immunohistochemical analysis of Ku70 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Ku70 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western-Blot-Analyse der Ku70-Expression in Wildtyp- (WT) und Knockdown- (KD) HeLa-Zelllysaten.
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