EVA1A Rabbit Polyclonal Antibody

Sicherheitsdatenblatt

Key features and details

Rabbit polyclonal antibody to EVA1A

Ziel: EVA1A
Quelle/Host: Kaninchen
Reaktivität: Human, Mouse, Rat
Klonalität: Polyklonal
Applications: WB
Konjugation: Unkonjugiert
Lagerung: bei -20°C

Marke:

CAT.NO. : APA13431

US$ Please choose

Größe:

50μL 100 μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Hintergrund

Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.

Bewerbung

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Übersicht

Beschreibung	Rabbit polyclonal antibody to EVA1A
Spezifität	Recognizes endogenous levels of EVA1A protein.
Antibody Type	Primary antibody
Immunogen	Recombinant fusion protein of human EVA1A
Reinigung	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 17 kD; Observed: 17 kD
Form/Puffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	FAM176A; TMEM166; Protein eva-1 homolog A; Protein FAM176A; Transmembrane protein 166
Gensymbol	EVA1A
Entrez Gene	84141(Human); 232146(Mouse)
SwissProt	Q9H8M9(Human); Q91WM6(Mouse)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Daten

Western blot analysis of EVA1A expression in A549 (A), U251 (B), rat lung (C) whole cell lysates. (Predicted band size: 17 kD; Observed band size: 17 kD)

Lagerung

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Hinweis

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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