Polyklonaler Kaninchen-Antikörper Ephrin A1
Sicherheitsdatenblatt
Hauptmerkmale und Details
- Ziel:
- Quelle/Host:
- Reaktivität:
- Klonalität:
- Anwendungen:
- Konjugation:
- Lagerung:
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Marke:
Produktdetails
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:100 |
IF/ICC | 1:50 - 1:200 |
Beschreibung | Polyklonaler Kaninchen-Antikörper gegen Ephrin A1 |
Spezifität | Erkennt endogene Mengen an Ephrin A1-Protein. |
Antikörpertyp | Primärer Antikörper |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Ephrin A1. The exact sequence is proprietary. |
Reinigung | Der Antikörper wurde durch Immunogenaffinitätschromatographie gereinigt. |
Molekulargewicht | Voraussichtlich: 23 kD; Beobachtet: 23 kD |
Form/Puffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Namen | EPLG1; LERK1; TNFAIP4; Ephrin-A1; EPH-related receptor tyrosine kinase ligand 1; LERK-1; Immediate early response protein B61; Tumor necrosis factor alpha-induced protein 4; TNF alpha-induced protein 4 |
Gensymbol | EFNA1 |
Entrez Gene | 1942 (Mensch); 13636(Maus); 94268(Ratte) |
SwissProt | P20827 (Mensch); P52793(Maus); P97553(Ratte) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Ephrin A1 expression in U87MG (A), MCF7 (B), CT26 (C), C6 (D) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 23 kD)
Immunohistochemical analysis of Ephrin A1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Ephrin A1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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