CPT1B Polyklonaler Kaninchen-Antikörper
Sicherheitsdatenblatt
Hauptmerkmale und Details
- Ziel:
- Quelle/Host:
- Reaktivität:
- Klonalität:
- Anwendungen:
- Konjugation:
- Lagerung:
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Marke:
Produktdetails
WB | 1:500 - 1:1000 |
IHC | 1:100 - 1:200 |
IF/ICC | 1:50 - 1:200 |
Beschreibung | Polyklonaler Kaninchen-Antikörper gegen CPT1B |
Spezifität | Erkennt endogene Mengen an CPT1B-Protein. |
Antikörpertyp | Primärer Antikörper |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CPT1B. The exact sequence is proprietary. |
Reinigung | Der Antikörper wurde durch Immunogenaffinitätschromatographie gereinigt. |
Molekulargewicht | Voraussichtlich: 87 kD; Beobachtet: 88 kD |
Form/Puffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Namen | KIAA1670; Carnitine O-palmitoyltransferase 1, muscle isoform; CPT1-M; Carnitine O-palmitoyltransferase I, muscle isoform; CPT I; CPTI-M; Carnitine palmitoyltransferase 1B; Carnitine palmitoyltransferase I-like protein |
Gensymbol | CPT1B |
Entrez Gene | 1375 (Mensch) |
SwissProt | Q92523 (Mensch) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of CPT1B expression in mouse heart (A), rat heart (B), PC3 (C) whole cell lysates. (Predicted band size: 87 kD; Observed band size: 88 kD)
Immunohistochemical analysis of CPT1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CPT1B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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