CD99 Mouse Monoclonal Antibody(C2615)

Sicherheitsdatenblatt

Key features and details

Mouse monoclonal to CD99

Ziel: CD99
Quelle/Host: Maus
Reaktivität: Mensch, Maus
Klonalität: Monoklonal
Anwendungen: WB, IHC, FC
Konjugation: Unkonjugiert
Lagerung: bei -20°C

Marke:

KAT.-NR. : AMA02227

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Größe:

50μL 100 μL

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Produktdetails

Hintergrund

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes .

Bewerbung

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:500
FC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Übersicht

Beschreibung	Mouse monoclonal to CD99
Spezifität	Recognizes endogenous levels of CD99 protein
Antikörpertyp	Primärer Antikörper
Immunogen	Recombinant fusion protein of human CD99 expressed in E. Coli
Reinigung	This antibody is purified through a protein G column.
Molekulargewicht	Predicted: 19 kD; Observed: 32 kD kD
Form/Puffer	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Namen	MIC,2; MIC,2X; MIC,2Y; CD99 antigen; 12E7; E2 antigen; Protein MIC,2; T-cell surface glycoprotein E2; CD99
Gensymbol	CD99
Entrez Gene	4267 (Mensch)
SwissProt	P14209(Human); Q8VCN6(Mouse)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Daten

Western blot analysis of CD99 expression in THP1 (A), U937 (B), MOLT4 (C), L1210 (D) whole cell lysates. (Predicted band size: 19 kD; Observed band size: 32 kD kD)

Immunohistochemical analysis of CD99 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of Jurkat cells using Anti-CD99 Antibody (green) and negative control (red).

Lagerung

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Hinweis

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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