CD4 Rabbit Monoclonal Antibody(C1646)
Sicherheitsdatenblatt
Key features and details
Recombinant rabbit monoclonal antibody to CD4
- Ziel:
- Quelle/Host:
- Reaktivität:
- Klonalität:
- Anwendungen:
- Konjugation:
- Lagerung:
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Marke:
KAT.-NR. : AMA01258
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Produktdetails
Hintergrund
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Bewerbung
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
Übersicht
Beschreibung | Recombinant rabbit monoclonal antibody to CD4 |
Spezifität | Recognizes endogenous levels of CD4 protein. |
Antikörpertyp | Primary antibody, Recombinant |
Immunogen | Recombinant fusion protein of human CD4. The exact sequence is proprietary. |
Reinigung | The antibody was purified by immunogen affinity chromatography. |
Molekulargewicht | Predicted: 51 kD; Observed: 55 kD |
Form/Puffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
Alternative Namen | T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4 |
Gensymbol | CD4 |
Entrez Gene | 920(Human); 12504(Mouse) |
SwissProt | P01730(Human); P06332(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Daten
Western blot analysis of CD4 expression in THP1 (A), mouse spleen (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 55 kD)
Lagerung
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Hinweis
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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