CD279 Rat Monoclonal Antibody(29F.1A12)
Sicherheitsdatenblatt
Key features and details
Rat monoclonal antibody to CD279
- Ziel:
- Quelle/Host:
- Reaktivität:
- Klonalität:
- Anwendungen:
- Konjugation:
- Lagerung:
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Marke:
CAT.NO. : AMA03702
US$ Please choose
US$ Please choose
Product Details
Hintergrund
Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity.
Bewerbung
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
IF/ICC | 1:50 - 1:200 |
FC | 1:500 - 1:2000 |
Übersicht
Beschreibung | Rat monoclonal antibody to CD279 |
Spezifität | Recognizes mouse CD279 |
Antikörpertyp | Primary antibody |
Immunogen | Mouse PD-1 transfected BHK cells |
Reinigung | The antibody was purified by affinity chromatography. |
Molecular Weight | N/A |
Form/Puffer | Rat IgG2a kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide. |
Alternative Names | PD1; Programmed cell death protein 1; Protein PD-1; hPD-1; CD279 |
Gensymbol | PDCD1 |
Entrez Gene | 18566(Maus) |
SwissProt | Q02242(Maus) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Daten
Lagerung
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Hinweis
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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