Tim17 Rabbit Polyclonal Antibody

ورقة البيانات

صحيفة بيانات سلامة المواد

Key features and details

Rabbit polyclonal antibody to Tim17

الهدف: تيم17
المصدر/المضيف: أرنب
التفاعل: الإنسان، الفأر، الجرذ
الاستنساخ: متعدد النسيلة
التطبيقات: البنك الدولي، IF/المحكمة الجنائية الدولية
الاقتران: غير مقترن
التخزين: عند-20 درجة مئوية

العلامة التجارية:

رقم القط. : أبا12904

دولار أمريكي يرجى الاختيار

الحجم:

50 ميكرولتر 100 ميكرولتر

Trail, Bulk size or Custom requests Please contact us

تفاصيل المنتج

الخلفية

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

التطبيق

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
إذا/المحكمة الجنائية الدولية	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

نظرة عامة

الوصف	Rabbit polyclonal antibody to Tim17
خصوصية	Recognizes endogenous levels of Tim17 protein.
نوع الجسم المضاد	الأجسام المضادة الأولية
مناعي	Recombinant fusion protein of human Tim17
تنقية	The antibody was purified by immunogen affinity chromatography.
الوزن الجزيئي	Predicted: 18 kD; Observed: 18 kD
النموذج/المخزن المؤقت	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
أسماء بديلة	MIMT17; TIM17; TIM17A; TIMM17; Mitochondrial import inner membrane translocase subunit Tim17-A; Inner membrane preprotein translocase Tim17a
رمز الجين	TIMM17A
إنتريز جين	10440(Human); 21854(Mouse); 54311(Rat)
سويس بروت	Q99595(Human); Q9Z0V8(Mouse); O35092(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

البيانات

Western blot analysis of Tim17 expression in A549 (A), MCF7 (B), mouse kidney (C), mouse heart (D) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 18 kD)

Immunofluorescent analysis of Tim17 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

التخزين

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ملاحظة

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

الأسئلة الشائعة

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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